An Artificial Enzyme Made by Covalent Grafting of an Fe<sup>II</sup> Complex into β‐Lactoglobulin: Molecular Chemistry, Oxidation Catalysis, and Reaction‐Intermediate Monitoring in a Protein
作者: Charlotte BuronDr. Katell Sénéchal‐DavidDr. Rémy RicouxJean‐Pierre Le CaërVincent GuérineauDr. Philippe MéjanelleDr. Régis GuillotDr. Christian HerreroProf. Jean‐Pierre MahyProf. Frédéric Banse
作者单位: 1Institut de Chimie Moléculaire et des Matériaux d'Orsay, Université Paris Sud, CNRS, 91405, Orsay CEDEX (France)
2 Centre de recherche de Gif, Institut de Chimie des Substances Naturelles CNRS, Avenue de la Terrasse, 91198 Gif‐sur‐Yvette Cedex (France)
3 Institut Universitaire de Technologie d'Orsay, Université Paris Sud, 91405, Orsay CEDEX (France)
刊名: Chemistry – A European Journal, 2015, Vol.21 (34), pp.12188-12193
来源数据库: Wiley Journal
DOI: 10.1002/chem.201501755
关键词: bioinorganic chemistrycoordination compoundsenzyme catalysisenzyme modelsiron
原始语种摘要: Abstract(#br)An artificial metalloenzyme based on the covalent grafting of a nonheme FeII polyazadentate complex into bovine β‐lactoglobulin has been prepared and characterized by using various spectroscopic techniques. Attachment of the FeII catalyst to the protein scaffold is shown to occur specifically at Cys121. In addition, spectrophotometric titration with cyanide ions based on the spin‐state conversion of the initial high spin ( S =2) FeII complex into a low spin ( S =0) one allows qualitative and quantitative characterization of the metal center’s first coordination sphere. This biohybrid catalyst activates hydrogen peroxide to oxidize thioanisole into phenylmethylsulfoxide as the sole product with an enantiomeric excess of up to 20 %....
全文获取路径: Wiley  (合作)

  • catalyst 催化剂
  • sulfoxidation 磺化氧化
  • catalysis 催化
  • catalytic 催化的
  • sup 吃晚饭
  • metalloenzyme 金属酶
  • coordination 配位
  • reaction 反酌
  • lactoglobulin 乳球朊
  • titration 滴定