The key long non‐coding RNA screening and validation between germinal vesicle and metaphase II of porcine oocyte in vitro maturation
作者: Yafei JiaoBangjun GaoGuodong WangHui LiJam Z. AhmedDandan ZhangSheng YeShulin LiuMengmei LiDeshun ShiBen Huang
作者单位: 1State Key Laboratory for Conservation and Utilization of Subtropical Agro‐Bioresources Guangxi University Nanning China
2 School of Animal Science and Technology Guangxi University Nanning China
3 Shanghai Key Laboratory of Regulatory Biology East China Normal University Shanghai China
刊名: Reproduction in Domestic Animals, 2020, Vol.55 (3), pp.351-363
来源数据库: Wiley Journal
DOI: 10.1111/rda.13620
关键词: In vitro maturationIncRNAsMRNAsOocytePigRNA‐seqScreening
原始语种摘要: Abstract(#br)Oocyte maturation plays a vitally important role in the reproduction of pigs. However, the roles of messenger RNAs (mRNAs) and long non‐coding RNAs (lncRNAs) in the developmental process of porcine oocyte maturation are still largely unclear. In this study, a transcriptome analysis of germinal vesicle (GV) and metaphase II (MII) of oocytes from Chinese Duroc pigs was performed. A total of 1,753,030 and 2,486 differentially expressed (DE) mRNAs, 22,811 and 9,868 DE lncRNAs were identified between GV and MII stages, respectively. Furthermore, functional enrichment analysis showed that the common DE mRNAs and DE lncRNAs during the process of maturation were mainly involved in biological process and cellular components. Our study provides new insights of the expression changes of...
全文获取路径: Wiley  (合作)

  • maturation 成熟
  • germinal 胚的
  • oocyte 卵母细胞
  • vesicle 气泡
  • metaphase 中期
  • coding 编码
  • contribute 贡献
  • porcine 猪的
  • reproduction 复制