Effects of cryopreservation on stallion sperm protamine messenger RNAs
作者: Ali KadivarNaser Shams EsfandabadiEhsan Dehghani NazhvaniAbolfazl ShiraziEbrahim Ahmadi
作者单位: 1Department of Clinical Science Faculty of Veterinary Medicine Shahrekord University Shahrekord Iran
2 Research Institute of Animal Embryo Technology Shahrekord University Shahrekord Iran
3 Theriogenology resident Department of Clinical Science Faculty of Veterinary Medicine Shahrekord University Shahrekord Iran
刊名: Reproduction in Domestic Animals, 2020, Vol.55 (3), pp.274-282
来源数据库: Wiley Journal
DOI: 10.1111/rda.13615
关键词: CryopreservationProtamine transcriptsSpermStallion
原始语种摘要: Abstract(#br)Protamines substitute DNA‐binding histones during late spermatogenesis in sperm nucleus. Stallion sperm contains all three variants of these arginine‐rich and positively charged nuclear proteins (P1, P2 and P3). Two variants of protamine‐2, that is P2 and P3, constitute approximately 15% of the entire protamine content. Also, the ratio of protamine‐1 to protamine‐2 varies among different mammalian species, and abnormal protamine ratios and protamine content are correlated with male infertility. In this study, changes in protamine mRNA abundance for all three protamines were investigated in stallion sperm during cryopreservation. Twelve ejaculates were collected from six sexually mature stallions. Sperm samples were divided into two parts for total mRNA extraction: one as...
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关键词翻译
关键词翻译
  • protamine 精蛋白
  • sperm 精子
  • stallion 公马
  • relative 相对的
  • transcriptase 转录酶
  • divided 分部联箱
  • evaluate 求...的值
  • expression 表示
  • mature 成孰的
  • mammalian 哺乳动物