Enrichment of HDL proteome and phospholipidome from human serum via IMAC/MOAC affinity
作者: Rabia JaveedDilshad HussainFahmida JabeenAdeela SaeedBatool FatimaMuhammad Naeem AshiqMuhammad Najam‐ul‐Haq
作者单位: 1Division of Analytical Chemistry, Institute of Chemical Sciences Bahauddin Zakariya University Multan Pakistan
2 Department of Chemistry The Women University Multan Pakistan
3 Department of Biochemistry Bahauddin Zakariya University Multan Pakistan
刊名: Biomedical Chromatography, 2020, Vol.34 (1), pp.n/a-n/a
来源数据库: Wiley Journal
DOI: 10.1002/bmc.4693
关键词: HDLHDL‐omics work flowHuman serumIntact apoproteinsLipoprotein extractionPhospholipids
原始语种摘要: Abstract(#br)High‐density lipoproteins (HDLs) have anti‐inflammatory and antioxidant properties and are potentially cardio‐protective. Defective HDL function is caused by alterations in both the proteome and lipidome of HDL particles. As potential biomarkers, the development of analytical methods is necessary for the enrichment of HDLs. Therefore, a method for selective enrichment of HDLs using immobilized metal ion affinity chromatography (IMAC) and metal oxide affinity chromatography (MOAC) is presented. SPE‐based isolation of HDLs from whole serum is adopted as an alternative to traditional ultracentrifugation methods followed by SDS–PAGE. The enrichment mechanism relies on isoelectric points of lipoproteins and metal oxide. Negatively charged lipoprotein particles interact with...
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关键词翻译
关键词翻译
  • affinity 亲和力
  • serum 血清
  • potentially 可能地
  • enrichment 富集
  • comparative 比较的
  • lipoprotein 脂蛋白
  • human 人的
  • extraction 提取
  • amyloid 淀粉状朊
  • metal 金属