αRep A3: A Versatile Artificial Scaffold for Metalloenzyme Design
作者: Thibault Di MeoDr. Wadih GhattasDr. Christian HerreroDr. Christophe VeloursProf. Philippe MinardProf. Jean‐Pierre MahyDr. Rémy RicouxDr. Agathe Urvoas
作者单位: 1Institut de Chimie Moléculaire et des Matériaux d'Orsay (ICMMO) UMR 8182 CNRS Univ. Paris Sud Université Paris‐Saclay Bât. 420, rue du Doyen Georges Poitou 91405 Orsay cedex France
2 Institute for Integrative Biology of the Cell (I2BC) CEA CNRS Univ. Paris‐Sud Université Paris‐Saclay 91198 Gif‐sur‐Yvette cedex France
刊名: Chemistry – A European Journal, 2017, Vol.23 (42), pp.10156-10166
来源数据库: Wiley Journal
DOI: 10.1002/chem.201701518
关键词: artificial enzymesDiels–Alder reactionenantioselective catalysisgreen chemistryprotein engineering
原始语种摘要: Abstract(#br)αRep refers to a new family of artificial proteins based on a thermostable α‐helical repeated motif. One of its members, αRep A3, forms a stable homo‐dimer with a wide cleft that is able to accommodate metal complexes and thus appears to be suitable for generating new artificial biocatalysts. Based on the crystal structure of αRep A3, two positions (F119 and Y26) were chosen, and independently changed into cysteine residues. A phenanthroline ligand was covalently attached to the unique cysteine residue of each protein variant, and the corresponding biohybrids were purified and characterized. Once mutated and coupled to phenanthroline, the protein remained folded and dimeric. Copper(II) was specifically bound by the two biohybrids with two different binding modes. Furthermore,...
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关键词翻译
关键词翻译
  • thermostable 耐热的
  • protein 蛋白质
  • artificial 人为的
  • purified 净化的
  • repeated 重复的
  • dimer 二聚物
  • cysteine 胖胱氨酸
  • reaction 反酌
  • attached 附加
  • catalysis 催化