Incorporation of Indole Significantly Improves the Transfection Efficiency of Guanidinium‐Containing Poly(Methacrylamide)s
作者: Ceren CokcaLeon ZartnerIlja TabujewDagmar FischerKalina Peneva
作者单位: 1Institute of Organic Chemistry and Macromolecular Chemistry (IOMC) Friedrich Schiller University Jena Lessingstraße 8 07743 Jena Germany
2 Institute of Pharmacy, Pharmaceutical Technology and Biopharmacy Friedrich Schiller University Jena Lessingstraße 8 07743 Jena Germany
3 Jena Center of Soft Matter Friedrich Schiller University Jena Philosophenweg 7 07743 Jena Germany
刊名: Macromolecular Rapid Communications, 2020, Vol.41 (6), pp.n/a-n/a
来源数据库: Wiley Journal
DOI: 10.1002/marc.201900668
关键词: Gene deliveryGuanidiniumIndoleMethacrylamide
原始语种摘要: Abstract(#br)A highly efficient transfection agent is reported that is based on terpolymer consisting of N ‐(2‐hydroxypropyl)methacrylamide (HPMA), N ‐(3‐guanidinopropyl) methacrylamide (GPMA), and N ‐(2‐indolethyl)methacrylamide monomers (IEMA) by analogy to the amphipathic cell‐penetrating peptides containing tryptophan and arginine residues. The incorporation of the indole‐bearing monomer leads to successful plasmid DNA condensation even at a nitrogen‐to‐phosphate (N/P) ratio of 1. The hydrodynamic diameter of polyplexes is determined to be below 200 nm for all N/P ratios. The transfection studies demonstrate a 200‐fold increase of the transgene expression in comparison to P(HPMA‐co‐GPMA) with the same guanidinium content. This study reveals the strong potential of the indole group as...
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  • methacrylamide 甲基丙烯酰胺
  • incorporation 掺合
  • transfection 转染
  • demonstrate 说明
  • efficient 有用的
  • phosphate 磷酸盐
  • pendant 下垂的
  • tryptophan 色氨酸
  • indole 吲哚
  • analogy 类似