Positional differences of intronic transposons in pAMT affect the pungency level in chili pepper through altered splicing efficiency
作者: Yoshiyuki TanakaTakaya AsanoYorika KanemitsuTanjuro GotoYuichi YoshidaKenichiro YasubaYuki MisawaSachie NakataniKenji Kobata
作者单位: 1Graduate School of Agriculture Kyoto University Sakyo‐ku Kyoto 606‐8502 Japan
2 Graduate School of Environmental and Life Science Okayama University Okayama 700‐8530 Japan
3 Graduate School of Pharmaceutical Sciences Josai University 1–1 Keyakidai Sakado Saitama 350‐0295 Japan
刊名: The Plant Journal, 2019, Vol.100 (4), pp.693-705
来源数据库: Wiley Journal
DOI: 10.1111/tpj.14462
关键词: Chili pepperCapsaicinoidCapsinoid
原始语种摘要: Summary(#br)Capsaicinoids are unique compounds that give chili pepper fruits their pungent taste. Capsaicinoid levels vary widely among pungent cultivars, which range from low pungency to extremely pungent. However, the molecular mechanisms underlying this quantitative variation have not been elucidated. Our previous study identified various loss‐of‐function alleles of the pAMT gene which led to low pungency. The mutations in these alleles are commonly defined by Tcc transposon insertion and its footprint. In this study, we identified two leaky pamt alleles ( pamt L1 and pamt L2) with different levels of putative aminotransferase (pAMT) activity. Notably, both alleles had a Tcc transposon insertion in intron 3, but the locations of the insertions within the intron were different. Genetic...
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关键词翻译
关键词翻译
  • pungency 刺激性
  • functional 功能的
  • pepper 胡椒
  • splicing 接合
  • chili 奇利风
  • splice 连接套管
  • intron 基因内区
  • sequencing 排序
  • alleles 对立形质
  • efficiency 效率