Enhancement of malate production through engineering of the periplasmic rTCA pathway in Escherichia coli
作者: Liang GuoFan ZhangCan ZhangGuipeng HuCong GaoXiulai ChenLiming Liu
作者单位: 1State Key Laboratory of Food Science and Technology Jiangnan University Wuxi China
2 Key Laboratory of Industrial Biotechnology Ministry of Education Jiangnan University Wuxi China
3 National Engineering Laboratory for Cereal Fermentation Technology Jiangnan University Wuxi China
刊名: Biotechnology and Bioengineering, 2018, Vol.115 (6), pp.1571-1580
来源数据库: Wiley Journal
DOI: 10.1002/bit.26580
关键词: Dual metabolic engineeringMalatePeriplasmic engineering
原始语种摘要: Abstract(#br)The compartmentalization of enzymes into organelles is a promising strategy for limiting metabolic crosstalk and improving pathway efficiency; however, prokaryotes are unicellular organisms that lack membrane‐bound organelles. To mimic this natural compartmentalization, we present here the targeting of the reductive tricarboxylic acid (rTCA) pathway to the periplasm to enhance the production of malate. A multigene combination knockout strategy was used to construct a phosphoenolpyruvate (PEP) pool. Then, the genes encoding phosphoenolpyruvate carboxykinase and malate dehydrogenase were combinatorially overexpressed to construct a cytoplasmic rTCA pathway for malate biosynthesis; however, the efficiency of malate production was low. To further enhance malate production, the...
全文获取路径: Wiley  (合作)

  • malate 苹果酸盐
  • engineering 工程
  • production 生产
  • tricarboxylic 三(个)羧基的
  • pathway 轨道
  • construct 建设
  • improving 改进
  • efficiency 效率
  • strategy 战略
  • enzymes 酶类