The role of the Fe‐S cluster in the sensory domain of nitrogenase transcriptional activator VnfA from Azotobacter vinelandii
作者: Hiroshi NakajimaNobuyuki TakataniKyohei YoshimitsuMitsuko ItohShigetoshi AonoYasuhiro TakahashiYoshihito Watanabe
作者单位: 1Department of Chemistry, Graduate School of Science, Nagoya University, Japan
2 Research Center of Materials Science, Nagoya University, Japan
3 Okazaki Institute for Integrative Biosciences, Japan
4 Division of Life Science, Graduate School of Science and Engineering, Saitama University, Japan
刊名: FEBS Journal, 2010, Vol.277 (3), pp.817-832
来源数据库: Wiley Journal
DOI: 10.1111/j.1742-4658.2009.07530.x
关键词: Azotobactor vinelandiiiron‐sulfur clusternitrogen fixationnitrogenasetranscriptional regulator
原始语种摘要: Transcriptional activator VnfA is required for the expression of a second nitrogenase system encoded in the vnfH and vnfDGK operons in Azotobacter vinelandii . In the present study, we have purified full‐length VnfA produced in E. coli as recombinant proteins ( Strep ‐tag attached and tag‐less proteins), enabling detailed characterization of VnfA for the first time. The EPR spectra of whole cells producing tag‐less VnfA (VnfA) show distinctive signals assignable to a 3Fe‐4S cluster in the oxidized form ([Fe3S4]+). Although aerobically purified VnfA shows no vestiges of any Fe‐S clusters, enzymatic reconstitution under anaerobic conditions reproduced [Fe3S4]+ dominantly in the protein. Additional spectroscopic evidence of...
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关键词翻译
关键词翻译
  • cluster 
  • purified 净化的
  • active 活动的
  • proteins 蛋白质
  • domain 领域
  • nitrogenase 固氮酶
  • attached 附加
  • less 更少的
  • required 必需
  • activator 活化剂