The role of the GAF and central domains of the transcriptional activator VnfA in Azotobacter vinelandii
作者: Kyohei YoshimitsuNobuyuki TakataniYukio MiuraYoshihito WatanabeHiroshi Nakajima
作者单位: 1Department of Chemistry, Graduate School of Science, Nagoya University, Japan
2 Graduate School of Bioagricultural Science, Nagoya University, Japan
3 Research Center of Materials Science, Nagoya University, Japan
刊名: FEBS Journal, 2011, Vol.278 (18), pp.3287-3297
来源数据库: Wiley Journal
DOI: 10.1111/j.1742-4658.2011.08245.x
关键词: Azotobacter vinelandiiiron‐sulfur clusternitrogenaseNTPase activitytranscriptional regulator
原始语种摘要: VnfA is a transcriptional activator that is required for the expression of the structural genes encoding nitrogenase‐2 in Azotobacter vinelandii . VnfA consists of three domains: an N‐terminal regulatory domain termed GAF, including a Cys‐rich motif; a central domain from the AAA+ family; and a C‐terminal domain for DNA binding. Previously, we reported that transcriptionally active VnfA harboring an Fe‐S cluster (presumably of the 3Fe‐4S type) as a prosthetic group and the Cys‐rich motif were possibly associated with coordination of the Fe‐S cluster. In the present study, we have investigated the roles of the GAF and central domains in the regulatory function of VnfA using truncated variants: ΔN15VnfA and ΔGAFVnfA that lack the N‐terminal 15 residues and whole GAF...
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  • central 中心的
  • domain 领域
  • cluster 
  • whereas 鉴于
  • accession 增加
  • activity 活度
  • motif 花纹图案
  • binding 装订
  • tetrameric 四部的
  • regulatory 控制的