Effect of budesonide on the methylation and mRNA expression of the insulin‐like growth factor 2 and c‐ myc genes in mouse lung tumors
作者: Lianhui TaoYingzhe LiWei WangPaula M. KramerWilliam T. GunningRonald A. LubetVernon E. SteeleMichael A. Pereira
作者单位: 1Department of Pathology, Medical College of Ohio, Toledo, Ohio
2 Chemopreventive Agent Development Research Group, Division of Cancer Prevention, National Cancer Institute, Bethesda, Maryland
3 Medical College of Ohio, Department of Pathology, Health Education Building, Room 202, 3055 Arlington Avenue, Toledo, Ohio 43614–5806.
刊名: Molecular Carcinogenesis, 2002, Vol.35 (2), pp.93-102
来源数据库: Wiley Journal
DOI: 10.1002/mc.10078
关键词: budesonidechemopreventionDNA methylationinsulin‐like growth factor 2c‐ myc
原始语种摘要: Abstract(#br)The use of surrogate end‐point biomarkers could help in the development of chemopreventive agents. To define potential surrogate end‐point biomarkers, the ability of budesonide to decrease mRNA expression of the insulin‐like growth factor‐2 ( Igf‐II ) and c‐ myc genes and to cause the remethylation of the genes was investigated in lung tumors. Lung tumors were induced in female strain A mice by administering i.p. 16 mg/kg vinyl carbamate for 2 consecutive wk or by a single dose of 100 mg/kg benzo[ a ]pyrene (B[ a ]P). Thirty‐four weeks later, the mice given vinyl carbamate received budesonide (0.6 or 2.4 mg/kg diet) for 7 d and then were killed. Mice were killed 24 wk after administration of B[ a ]P. The mRNA expression of the Igf‐II and c‐ myc genes was increased in lung...
全文获取路径: Wiley  (合作)

  • expression 表示
  • mouse 小鼠
  • myc 真菌
  • insulin 胰岛素
  • factor 因素
  • growth 生长
  • methylation 甲基化酌
  • numbers 数目
  • sequencing 排序
  • increased 增加