Independent mobility of proteins and lipids in the plasma membrane of E scherichia coli
作者: Anja NenningerGiulia MastroianniAlexander RobsonTchern LennQuan XueMark C. LeakeConrad W. Mullineaux
作者单位: 1School of Biological and Chemical Sciences Queen Mary University of London Mile End Road London E1 4NS UK
2 Clarendon Laboratory Department of Physics University of Oxford Parks Road Oxford OX1 3PU UK
3 Biological Physical Sciences Institute (BPSI) Departments of Physics of Biology University of York York YO10 5DD UK
刊名: Molecular Microbiology, 2014, Vol.92 (5), pp.1142-1153
来源数据库: Wiley Journal
DOI: 10.1111/mmi.12619
原始语种摘要: Summary(#br)Fluidity is essential for many biological membrane functions. The basis for understanding membrane structure remains the classic S inger‐ N icolson model, in which proteins are embedded within a fluid lipid bilayer and able to diffuse laterally within a sea of lipid. Here we report lipid and protein diffusion in the plasma membrane of live cells of the bacterium E scherichia coli , using F luorescence R ecovery after P hotobleaching ( FRAP ) and T otal I nternal R eflection F luorescence ( TIRF ) microscopy to measure lateral diffusion coefficients. Lipid and protein mobility within the membrane were probed by visualizing an artificial fluorescent lipid and a simple model membrane protein consisting of a single membrane‐spanning alpha‐helix with a G reen F luorescent P rotein...
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