The FGF ‐1‐specific single‐chain antibody scFv1C9 effectively inhibits breast cancer tumour growth and metastasis
作者: Hengliang ShiChunling FuWei WangYu LiShuang DuRangjuan CaoJingying ChenDong SunZhongyu ZhangXingzhi WangXiaojuan Zhu
作者单位: 1Key Laboratory of Molecular Epigenetics of the Ministry of Education Institute of Cytology and Genetics Northeast Normal University Changchun China
2 Department of Neurosurgery Affiliated Hospital of Xuzhou Medical College Xuzhou China
3 Department of Hematology Affiliated Hospital of Xuzhou Medical College Xuzhou China
刊名: Journal of Cellular and Molecular Medicine, 2014, Vol.18 (10), pp.2061-2070
来源数据库: Wiley Journal
DOI: 10.1111/jcmm.12371
关键词: FGF ‐1lung metastasisscFv1C9tumour growthtumourigenesis
原始语种摘要: Abstract(#br)Immunotherapy mediated by recombinant antibodies is an effective therapeutic strategy for a variety of cancers. In a previous study, we demonstrated that the fibroblast growth factor 1 ( FGF ‐1)‐specific recombinant antibody scFv1C9 arrests the cell cycle at the G0/G1 transition by blocking the intracrine FGF ‐1 pathway in breast cancer cells. Here, we further show that the overexpression of scFv1C9 in MCF ‐7 and MDA ‐ MB ‐231 breast cancer cells by lentiviral infection resulted in decreased tumourigenicity, tumour growth and lung metastasis through FGF ‐1 neutralization. We found that scFv1C9 resulted in the up‐regulation of p21, which in turn inhibited the expression of CDK 2 and blocked cell cycle progression. To explore the potential role of scFv1C9 in vivo , we delivered...
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