Cytochrome C oxidase subunit I barcodes provide an efficient tool for Jinqian Baihua She (Bungarus parvus) authentication
作者: Zhi ChaoJing LiaoZhenbiao LiangSuhua HuangLiang ZhangJunde Li
作者单位: 1Department of Pharmacognosy, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou
2Department of Pharmacognosy, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou; Beijing Royal Intergrative Medicine Hospital, Beijing
3Department of Pharmacognosy, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou; Department of Pharmacy, Zhongshan People′s Hospital, Zhongshan
4South China Institute of Endangered Animals, Guangzhou
5Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing
刊名: Pharmacognosy Magazine, 2014, Vol.10 (40), pp.449-457
来源数据库: Wolters Kluwer Journal
DOI: 10.4103/0973-1296.141816
关键词: Bungarus multicinctuscytochrome C oxidase subunit IDNA barcodesidentification
原始语种摘要: Objective: To test the feasibility of DNA barcoding for accurate identification of Jinqian Baihua She and its adulterants. Materials and Methods: Standard cytochrome C oxidase subunit I (COI) gene fragments were sequenced for DNA barcoding of 39 samples from 9 snake species, including Bungarus multicinctus, the officially recognized origin animal by Chinese Pharmacopoeia, and other 8 adulterate species. The aligned sequences, 658 base pairs in length, were analyzed for divergence using the Kimura-2-parameter (K2P) distance model with MEGA5.0. Results: The mean intraspecific K2P distance was 0.0103 and the average interspecific genetic distance was 0.2178 in B. multicinctus, far greater than the minimal interspecific genetic distance of 0.027 recommended for species identification. A...
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影响因子:1.525 (2012)

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关键词翻译
关键词翻译
  • authentication 确认
  • adulterants 掺杂物
  • provide 规定
  • identification 辨认
  • oxidase 氧化酶
  • interspecific 种间的
  • subunit 亚单位
  • species 
  • accurate 精确的
  • snake