Time course analysis of sensory axon regeneration in vivo by directly tracing regenerating axons
作者: Yan GaoYi-Wen HuRun-Shan DuanShu-Guang YangFeng-Quan ZhouRui-Ying Wang
作者单位: 1Guilin Medical University, Guilin, Guangxi Zhuang Autonomous Region
2Department of Orthopedic Surgery, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi Zhuang Autonomous Region
3Department of Orthopedics, Henan Provincial People';s Hospital, People';s Hospital of Zhengzhou University, Zhengzhou, Henan Province
4Department of Orthopedic Surgery, Johns Hopkins University School of Medicine, Baltimore, MD
刊名: Neural Regeneration Research, 2020, Vol.15 (6), pp.1160-1165
来源数据库: Wolters Kluwer Journal
DOI: 10.4103/1673-5374.270315
原始语种摘要: Most current studies quantify axon regeneration by immunostaining regeneration-associated proteins, representing indirect measurement of axon lengths from both sensory neurons in the dorsal root ganglia and motor neurons in the spinal cord. Our recently developed method of in vivo electroporation of plasmid DNA encoding for enhanced green fluorescent protein into adult sensory neurons in the dorsal root ganglia provides a way to directly and specifically measure regenerating sensory axon lengths in whole-mount nerves. A mouse model of sciatic nerve compression was established by squeezing the sciatic nerve with tweezers. Plasmid DNA carrying enhanced green fluorescent protein was transfected by ipsilateral dorsal root ganglion electroporation 2 or 3 days before injury. Fluorescence...
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影响因子:0.144 (2012)

  • fluorescent 萤光的
  • regeneration 再生
  • sensory 传感
  • dorsal 背面的
  • expression 表示
  • sciatic 坐骨的
  • protein 蛋白质
  • nerve 叶脉
  • observed 观察到的
  • regenerating 再生