Hybrid three-dimensional (3D) bioprinting of retina equivalent for ocular research
作者: Pujiang Shi Tan Yong Sheng Edgar Wai Yee Yeong Augustinus Laude
刊名: International Journal of Bioprinting, 2017, Vol.3 (2)
来源数据库: Whioce Publishing Pte. Ltd
DOI: 10.18063/IJB.2017.02.008
关键词: retinabioprintingage-related macular degenerationtissue engineering
原始语种摘要: In this article, a hybrid retina construct was created via three-dimensional (3D) bioprinting technology. The construct was composed of a PCL ultrathin membrane, ARPE-19 cell monolayer and Y79 cell-laden alginate/pluronic bioink. 3D bioprinting technology was applied herein to deliver the ARPE-19 cells and Y79 cell-laden bioink to ensure homogeneous ARPE-19 cell seeding; subsequently, two distinctive Y79 cell-seeding patterns were bioprinted on top of the ARPE-19 cell monolayer. The bioprinted ARPE-19 cells were evaluated by prestoblue assay, F-actin, and hematoxylin/eosin (HE) staining, and then the cells were observed under laser scanning and invert microscopy for 14 days. The Y79 cells in alginate/pluronic bioink after bioprinting had been closely monitored for 7 days. Live/dead assay...
全文获取路径: Whioce Publishing  (合作)
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关键词翻译
关键词翻译
  • seeding 幼苗
  • retina 视网膜
  • construct 建设
  • investigate 
  • scanning 扫描
  • dimensional 量纲的
  • three 
  • cells 麻风细胞
  • monolayer 单分子层
  • pluronic 一种非离子型表面活性剂