The Application of Realtime Fluorescence Quantitative PCR for Prenatal Screening of Group B Streptococcal Infections
作者: Changzhi XuDonglin ZhuZhizhi XieYun Xi
作者单位: 1Department of Laboratory Medicine, The Third Affliated Hospital of Sun Yat-sen University,china
刊名: Journal of Advances in Medicine Science, 2019, Vol.2 (3)
来源数据库: Bilingual Publishing Co.
DOI: 10.30564/jams.v2i3.669
关键词: Streptococcus BFluorescence quantitative PCRBacterial cultureGene sequencing
原始语种摘要: Objective: In the prenatal screening, several different methods were used to detect the presence of group B streptococcus (GBS) infection, in this assay, the diagnostic value and clinical signifcance of the application of realtime fluorescent PCR were explored. Methods: A total of 86 women with 35-37 weeks pregnancy were enrolled, vaginal secretion samples were collected. Fluorescence PCR, bacterial culture and gene sequencing were used to detect whether there was GBS infection, and the results obtained were compared and analyzed. Results: 10 subjects were detected to be positive for GBS by fluorescence PCR (the positive rate was 11.6%), however, only 4 cases were positive for GBS by bacterial culture method (the positive rate was 4.7%). There was a statistically signifcant difference in...
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关键词翻译
关键词翻译
  • Fluorescence 荧光
  • sequencing 排序
  • detect 探测
  • PCR Page Control Register
  • positive 阳片
  • bacterial 细菌的
  • screening 筛分
  • prenatal 产前的
  • several 各自
  • culture