COMPARISON OF RNA EXTRACTION METHODS FOR TRANSCRIPT ANALYSIS FROM THE PSYCHROPHILIC YEAST, Glaciozyma antarctica
作者: Bharudin, I Zaki. N.Z Abu Bakar, F.D Mahadi, N.M Najimudin, N Illias, R.M.Murad, A.M.A.
刊名: Malaysian Applied Biology, 2014, Vol.43 (2)
来源数据库: Malaysian Society of Applied Biology
关键词: Glaciozyma antarcticaRNA extractioncDNA librarypsychrophiles
原始语种摘要: To survive in extremely cold environments, psychrophilic microorganisms produce exopolysaccharides (EPS), which are carbohydrate polymers that constitute a substantial component of the extracellular polymers surrounding cells of these microorganisms. EPS can interfere with RNA extraction and decrease the purity of the RNA extracted from EPS producing microorganisms. In this work, six commercial RNA extraction kits and two published protocols for RNA extraction were evaluated for total RNA extraction from the psychrophilic yeast, Glaciozyma antarctica. All the protocols were optimised to obtain the highest quality of total RNA. The results show that all of the tested commercial kits and the tested conventional methods yielded RNA from G. antarctica, albeit with varying quality. The...
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关键词翻译
关键词翻译
  • RNA ROYAL NEPAL AIRLINES CORP.
  • transcriptase 转录酶
  • extraction 提取
  • desaturase 去饱和酶
  • highest 最高的
  • produce 制作
  • psychrophilic 喜低温的
  • tested 已试验的
  • expression 表示
  • cDNA 互补 DNA