Liver is a remarkable vital organ, it is responsible for metabolizing the different food elements, filtering and detoxifying poisons in the blood to remove numerous toxic compounds. Liver affections are essentially caused by harmful chemicals (certain anti-infection, paracetamol, carbon tetrachloride, aflatoxins, per oxidized oil and so on.), overabundance utilization of liquor, immune system issue and disease. Up till now, orthotropic liver transplantation is the most powerful treatment of liver infections. Because of the deficiency of donors, high costs, the number of patients who can profit by this methodology is extremely constrained. Recently, cell based treatments have been explored as other option to entire liver transplantation. Thirty rats were separated into three equivalent... groups (n=10, each). Group 1: control; Group 2: was infused peritoneally with acetaminophen (300 mg/kg); Group 3: was injected intra peritoneal with acetaminophen (300 mg/kg), then injected intra-venous with mesenchymal stromal stem cells (1 million BM-MSCs/kg). Blood samples for estimation of liver functions, and liver samples for detection of homing labeled MSCs with red fluorescent cell linker dye (PKH26), gene expression of matrix metalopeptidase (MMP2), tissue inhibitor metalopeptidase (TIMP) and histopathological examination were collected. The infusion of BM-MSCs enhanced liver capacities test, MMP2 and TIMP. Histopathological examination of liver tissue demonstrated critical antifibrotic impact, improvement in the hepatic structure as compared to the second group. It could be concluded that BM-MSC is effective in treatment of liver damage by upgrading hepatocyte recovery through enhancing the liver stress and inflammatory signaling by improving MMP2 and TIMP, besides it has a significant antifibrotic effect. The MSCs stem cell is an attractive cell source for regenerative medicine.