Phenogenotyping of Closely Related Methicillin Resistant Staphylococcus aureus Isolated from Milk and Meat Products
作者: Adel A. M. Ahmad
刊名: Zagazig Veterinary journal, 2017, Vol.45 (4)
来源数据库: Zagazig Veterinary Journal
DOI: DOI: 10.21608/ZVJZ.2017.7870
关键词: MRSACoa geneCoa polymorphismPCR-RFLP
原始语种摘要: Differentiation of closely related methicillin resistant staphylococcus aureus (MRSA) isolated from food chain is required for epidemiological and food safety studies. This study provided evidence for using coagulase (coa) gene sequence repeats as a powerful diagnostic tool for differentiation of closely MRSA isolated from different sources. Staphylococcal species were isolated from milk, meat, and meat-products. Staphylococcus aureus strains with identical antibiograms were differentiated using enterotoxin genotyping, coa gene polymorphism, the patterns of polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) of coa and nucleotides sequencing of coa gene-PCR products. Nine CoPs and MRSA strains were closely related and could not be differentiated by antibiogram....
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  • sequencing 排序
  • nucleotide 核苷酸
  • closely 精密地
  • related 有关的
  • enterotoxin 肠毒素
  • polymorphism 多晶形
  • chain 
  • powerful 强大的
  • region 地域
  • isolated 隔离