Development and validation of stability indicating HPTLC method for estimation of dextromethorphan hydrobromide
作者: Santosh V Gandhi Shubhangi K. Dyandyan
刊名: Journal of Applied pharmaceutical Research, 2017, Vol.5 (3)
来源数据库: Creative Pharma Assent
DOI: 10.18231/2348-0335.2017.0004
关键词: High performance thin layer chromatography (HPTLC)Dextromethorphan hydrobromideStability indicatingValidation
原始语种摘要: A simple, sensitive and accurate stability indicating HPTLC method has been developed and validated for estimation of Dextromethorphan hydrobromide in bulk and pharmaceutical dosage form. The drug was spotted on precoated silica gel 60 F254 aluminum plates using Toluene: Methanol: Triethylamine (8.5:1:0.5 v/v/v) as mobile phase. The retention factor (Rf) was found to be 0.60±1.92. The detection of band was carried at 225 nm. The drug was subjected to different stress conditions like acid, base, neutral hydrolysis, oxidation, thermal degradation and photolysis. The method was successfully validated according to ICH guidelines Q2 (R1). The data of linear regression analysis indicated a good linear relationship over the concentration range of 2000-20000 ng/band with correlation coefficient...
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关键词翻译
关键词翻译
  • hydrobromide 氢溴化物
  • indicating 指示
  • estimation 估计
  • pharmaceutical 
  • found 创立
  • HPTLC 高效薄层色谱法
  • accurate 精确的
  • dosage 放射剂量
  • analysis 分析
  • sensitive 灵敏的