Use of genetically modified muscle and fat grafts to repair defects in bone and cartilage
作者: C H EvansF&hyphenJ LiuV GlattJ A HoylandC Kirker&hyphenHeadA WalshO BetzJ W WellsV BetzR M PorterF A SaadL C GerstenfeldT A EinhornM B HarrisM S Vrahas
作者单位: 1Center for Molecular Orthopaedics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA,
刊名: Eur Cell Mater, 2009, Vol.18 , pp.96-111
来源数据库: European Cells and Materials (eCM)
DOI: 10.22203/eCM.v018a09
原始语种摘要: We report a novel technology for the rapid healing of large osseous and chondral defects, based upon the genetic modification of autologous skeletal muscle and fat grafts. These tissues were selected because they not only possess mesenchymal progenitor cells and scaffolding properties, but also can be biopsied, genetically modified and returned to the patient in a single operative session. First generation adenovirus vector carrying cDNA encoding human bone morphogenetic protein‐2 (Ad.BMP‐2) was used for gene transfer to biopsies of muscle and fat. To assess bone healing, the genetically modified („gene activated”) tissues were implanted into 5mm‐long critical size, mid‐diaphyseal, stabilized defects in the femora of Fischer...
全文获取路径: European Cells and Materials (eCM) 
影响因子:4.558 (2012)

  • muscle 肌肉
  • cartilage 软骨
  • hyphen 连字符
  • chondral 软骨的
  • diaphyseal 骨干的
  • ossification 骨化
  • healing 加硫
  • morphogenetic 地貌形成作用的
  • donor 给予体
  • operative 有法律效力