Cultivation of auricular chondrocytes in poly(ethylene glycol)/poly(ε-caprolactone) hydrogel for tracheal cartilage tissue engineering in a rabbit model
作者: C S ChangC Y YangH Y HsiaoL ChenI M ChuM H ChengC H Tsao
作者单位: 1Division of Reconstructive Microsurgery, Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, 5 Fu-Hsing Street, Kweishan, Taoyuan 33305,
刊名: Eur Cell Mater, 2018, Vol.35 , pp.350-364
来源数据库: European Cells and Materials (eCM)
DOI: 10.22203/eCM.v035a24
原始语种摘要: Tissue engineering has the potential to overcome the limitations of tracheal reconstruction. To tissue-engineer a tracheal cartilage, auricular chondrocytes were encapsulated in a photocurable poly(ethylene glycol)/poly(ε-caprolactone) (PEG/PCL) hydrogel. Chondrogenic genes, including Sox9, Acan and Col2a1, were up-regulated in auricular chondrocytes after 2 weeks of in vitro cultivation in the PEG/PCL hydrogel. Co-cultivation of 70 % auricular chondrocytes and 30 % bone marrow mesenchymal stem cells (BMSCs) accelerated the chondrogenic genes’ expression in the PEG/PCL hydrogel. Cartilaginous matrix markers, including proteoglycans and collagen type II, were detected in the chondrocytes-encapsulated PEG/PCL hydrogel after 4 weeks of in vitro cultivation. The higher expression level of...
全文获取路径: European Cells and Materials (eCM)  (合作)
影响因子:4.558 (2012)

  • hydrogel 水凝胶
  • caprolactone 已内酯
  • poly 
  • auricular 耳的
  • tracheal 气管的
  • engineering 工程
  • cultivation 栽培
  • PCL Packet Control Layer
  • cartilage 软骨
  • ingrowth 向内长