Thermoreversible hyaluronan-hydrogel and autologous nucleus pulposus cell delivery regenerates human intervertebral discs in an ex vivo, physiological organ culture model
作者: D H RosenzweigR FairagA P MathieuL LiD EglinM D’EsteT SteffenM H WeberJ A OuelletL Haglund
作者单位: 1Department of Surgery, Montreal General Hospital, Room C10.148.2, 1650 Cedar Ave, Montreal, QC H3G 1A4, Canada.lisbet.haglund@mcgill.ca
刊名: Eur Cell Mater, 2018, Vol.36 , pp.200-217
来源数据库: European Cells and Materials (eCM)
DOI: 10.22203/eCM.v036a15
原始语种摘要: Numerous studies show promise for cell-based tissue engineering strategies aiming to repair painful intervertebral disc (IVD) degeneration. However, clinical translation to human IVD repair is slow. In the present study, the regenerative potential of an autologous nucleus pulposus (NP)-cell-seeded thermoresponsive hyaluronic acid hydrogel in human lumbar IVDs was assessed under physiological conditions. First, agarose-encased in vitro constructs were developed, showing greater than 90 % NP cell viability and high proteoglycan deposition within HA-pNIPAM hydrogels following 3 weeks of dynamic loading. Second, a bovine-induced IVD degeneration model was used to optimise and validate T1ρ magnetic resonance imaging (MRI) for detection of changes in proteoglycan content in isolated intact...
全文获取路径: European Cells and Materials (eCM)  (合作)
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影响因子:4.558 (2012)

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关键词翻译
关键词翻译
  • intervertebral 椎间的
  • hydrogel 水凝胶
  • autologous 自体的
  • human 人的
  • lumbar 腰的
  • degenerative 退化的
  • painful 痛苦的
  • physiological 生理的
  • intact 完整的
  • culture