Intraoperative engineering of osteogenic grafts combining freshly harvested, human adipose-derived cells and physiological doses of bone morphogenetic protein-2
作者: A MehrkensF SaxerS GüvenW HoffmannA M MüllerM JakobF E WeberI MartinA Scherberich
作者单位: 1Institute for Surgical Research & Hospital Management, University Hospital Basel, Hebelstrasse 20, ZLF, Room 405, 4031 Basel,
刊名: Eur Cell Mater, 2012, Vol.24 , pp.308-319
来源数据库: European Cells and Materials (eCM)
DOI: 10.22203/eCM.v024a22
原始语种摘要: Engineered osteogenic constructs for bone repair typically involve complex and costly processes for cell expansion. Adipose tissue includes mesenchymal precursors in large amounts, in principle allowing for an intraoperative production of osteogenic grafts and their immediate implantation. However, stromal vascular fraction (SVF) cells from adipose tissue were reported to require a molecular trigger to differentiate into functional osteoblasts. The present study tested whether physiological doses of recombinant human BMP-2 (rhBMP-2) could induce freshly harvested human SVF cells to generate ectopic bone tissue. Enzymatically dissociated SVF cells from 7 healthy donors (1 x 106 or 4 x 106) were immediately embedded in a fibrin gel with or without 250 ng rhBMP-2, mixed with porous silicated...
全文获取路径: European Cells and Materials (eCM) 
影响因子:4.558 (2012)

  • osteogenic 成骨的
  • cells 麻风细胞
  • adipose 脂肪的
  • human 人的
  • morphogenetic 地貌形成作用的
  • freshly 
  • constructs 建筑图纸生成系统
  • derived 导生的
  • lineage 血统
  • mesenchymal 间叶细胞的