Localisation of mineralised tissue in a complex spinner flask environment correlates with predicted wall shear stress level localisation
作者: J MelkeF ZhaoB van RietbergenK ItoS Hofmann
作者单位: 1Eindhoven University of Technology, PO Box 513, 5600 MB Eindhoven, the Netherlands.S.Hofmann.Boss@tue.nl
刊名: Eur Cell Mater, 2018, Vol.36 , pp.57-68
来源数据库: European Cells and Materials (eCM)
DOI: 10.22203/eCM.v036a05
原始语种摘要: Spinner flask bioreactors have often been employed for bone tissue engineering. However, the reasons for their success in facilitating bone growth remain inconclusive. It was hypothesised that engineered bone tissue formation can be attributed to mechanical stimuli, which can be predicted in the tissue engineered construct. To test the hypothesis and draw conclusions as to how mechanical stimulation affects cell behaviour, a multi- disciplinary approach using cell culture experiments and computational fluid dynamics (CFD) to simulate the complex flow within the spinner flask and scaffold was employed. Micro-computed tomography and histology showed that statically cultured human bone marrow derived stromal cells on silk fibroin scaffolds did not form extracellular matrix (ECM) or deposit...
全文获取路径: European Cells and Materials (eCM) 
影响因子:4.558 (2012)

  • bottom 底壁
  • spinner 旋涂器
  • predicted 预先计算的
  • complex 超群
  • disciplinary 规程
  • tissue 组织
  • scaffold 脚手架
  • flask 烧瓶
  • shear 切变
  • computational 计算的