High glucose exposure promotes proliferation and in vivo network formation of adipose-tissue-derived microvascular fragments
作者: M W LaschkeM S SeifertC ScheuerE KontaxiW MetzgerM D Menger
作者单位: 1Institute for Clinical and Experimental Surgery, Saarland University, D-66421 Homburg/Saar, Germany.matthias.laschke@uks.eu
刊名: Eur Cell Mater, 2019, Vol.38 , pp.188-200
来源数据库: European Cells and Materials (eCM)
DOI: 10.22203/eCM.v038a13
原始语种摘要: High glucose concentrations have been shown to activate endothelial cells and promote angiogenesis. In the present study, it was investigated whether high glucose concentrations could improve the vascularisation capacity of adipose-tissue-derived microvascular fragments (ad-MVF). Ad-MVF were isolated from the epididymal fat pads of donor mice and cultivated for 24 h in University of Wisconsin (UW) solution supplemented with vehicle or 30 mM glucose. Protein expression, morphology, viability and proliferation of the cultivated ad-MVF were analysed by means of proteome profiler mouse angiogenesis array, scanning electron microscopy and immunohistochemistry. Additional cultivated ad-MVF were seeded on to collagen-glycosaminoglycan scaffolds to study their in vivo vascularisation capacity in...
全文获取路径: European Cells and Materials (eCM) 
影响因子:4.558 (2012)

  • glucose 葡萄糖
  • adipose 脂肪的
  • exposure 暴露
  • microvascular 微脉管(直径小于0.3 毫米的微血管)的
  • tissue 组织
  • derived 导生的
  • cultivated 栽培的
  • proliferation 增生
  • protein 蛋白质
  • improve 改进