YAP/TAZ regulates the expression of proteoglycan 4 and tenascin C in superficial-zone chondrocytes
作者: E DelveV CoS C RegmiJ ParrenoT A SchmidtR A Kandel
作者单位: 1Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, 600 University Avenue, Suite 6-500, Toronto, M5G 1X5, Ontario, Canada.rita.kandel@sinaihealthsystem.ca
刊名: Eur Cell Mater, 2020, Vol.39 , pp.48-64
来源数据库: European Cells and Materials (eCM)
DOI: 10.22203/eCM.v039a03
原始语种摘要: The roles of cell division control protein 42 homologue (CDC42) and actin polymerisation in regulating the phenotype of superficial-zone chondrocytes (SZCs) have been demonstrated in vitro; however, the signalling pathway(s) downstream have yet to be fully elucidated. The study hypothesis was that Yes-associated protein (YAP) and transcriptional co-activator with PDZ-binding motif (TAZ) act downstream to regulate proteoglycan 4 (PRG4) and tenascin C (TNC). Bovine SZCs grown in monolayer were treated with ML141 (CDC42 inhibitor) or the actin depolymerising agents, latrunculin B and cytochalasin D, to determine the effect on YAP/TAZ. Verteporfin (YAP/TAZ inhibitor) and YAP/TAZ siRNA-mediated knockdown were used to determine their role in regulating PRG4 and TNC. ML141 treatment reduced...
全文获取路径: European Cells and Materials (eCM) 
影响因子:4.558 (2012)

  • expression 表示
  • YAP Yaw and Pitch
  • knockdown 击倒
  • cytochalasin 细胞松弛素
  • superficial 表面的
  • nuclear 原子核的
  • protein 蛋白质
  • regulated 规定
  • downstream 顺聊
  • demonstrated 探明的储量