Paracrine effects of living human bone particles on the osteogenic differentiation of mesenchymal stem cells
作者: A Atasoy-ZeybekA IvkovicT BeyzadeogluA OnaC H EvansG T Kose
作者单位: 1Rehabilitation Medicine Research Centre, Mayo Clinic, 200 First Street SW, Rochester, MN, 55905,
刊名: Eur Cell Mater, 2019, Vol.38 , pp.14-22
来源数据库: European Cells and Materials (eCM)
DOI: 10.22203/eCM.v038a02
原始语种摘要: Bone autografting remains the clinical model of choice for resolving problematic fractures. The precise mechanisms through which the autograft promotes bone healing are unknown. The present study examined the hypothesis that cells within the autograft secrete osteogenic factors promoting the differentiation of mesenchymal stem cells (MSCs) into osteoblasts. Particles of human bone (“chips”) were recovered at the time of joint replacement surgery and placed in culture. Then, conditioned media were added to cultures of human, adipose-derived MSCs under both basal and osteogenic conditions. Contrary to expectation, medium conditioned by bone chips reduced the expression of alkaline phosphatase and strongly inhibited mineral deposition by MSCs cultured in osteogenic medium. Real time PCR...
全文获取路径: European Cells and Materials (eCM) 
影响因子:4.558 (2012)

  • osteogenic 成骨的
  • mesenchymal 间叶细胞的
  • cells 麻风细胞
  • human 人的
  • autograft 自体组织移植片
  • conditioned 经过调节的
  • cultured 人工培养的
  • expression 表示
  • chips 碎石
  • phosphatase 磷酸酶