Nondestructive fluorescence lifetime imaging and time-resolved fluorescence spectroscopy detect cartilage matrix depletion and correlate with mechanical properties
作者: A K HaudenschildB E SherlockX ZhouJ C HuJ K LeachL MarcuK A Athanasiou
作者单位: 1University of California Irvine, 3418 Engineering Hall, Irvine, CA 92697,
刊名: Eur Cell Mater, 2018, Vol.36 , pp.30-43
来源数据库: European Cells and Materials (eCM)
DOI: 10.22203/eCM.v036a03
原始语种摘要: Tissue engineers utilize a battery of expensive, time-consuming and destructive techniques to assess the composition and function of engineered tissues. A nondestructive solution to monitor tissue maturation would reduce costs and accelerate product development. As a first step toward this goal, two nondestructive, label-free optical techniques, namely multispectral fluorescent lifetime imaging (FLIm) and time-resolved fluorescence spectroscopy (TRFS), were investigated for their potential in evaluating the biochemical and mechanical properties of articular cartilage.Enzymatic treatments were utilized to selectively deplete cartilage of either collagen or proteoglycan, to produce a range of matrix compositions. Samples were assessed for their optical properties using a fiber-coupled...
全文获取路径: European Cells and Materials (eCM)  (合作)
影响因子:4.558 (2012)

  • mechanical 机械的
  • properties 道具
  • fluorescence 荧光
  • their 他们的
  • matrix 石基
  • cartilage 软骨
  • lifetime 寿命
  • development 开发
  • nondestructive 不破损的
  • spectroscopy 分光学