Lentiviral-based reporter constructs for profiling chondrogenic activity in primary equine cell populations
作者: A Martin-PenaR M PorterG PlumtonT M McCarrelA J MortonM V GuijarroS C GhivizzaniB SharmaG D Palmer
作者单位: 1IDepartment of Orthopaedics and Rehabilitation, University of Florida, 1600 SW Archer Rd, MSB, M2-235, Gainesville, FL, 32610, USA.glynpal@gmail.com
刊名: Eur Cell Mater, 2018, Vol.36 , pp.156-170
来源数据库: European Cells and Materials (eCM)
DOI: 10.22203/eCM.v036a12
原始语种摘要: Successful clinical translation of mesenchymal stem cell (MSC)-based therapies for cartilage repair will likely require the implementation of standardised protocols and broadly applicable tools to facilitate the comparisons among cell types and chondroinduction methods. The present study investigated the utility of recombinant lentiviral reporter vectors as reliable tools for comparing chondrogenic potential among primary cell populations and distinguishing cellular-level variations of chondrogenic activity in widely used three-dimensional (3D) culture systems. Primary equine MSCs and chondrocytes were transduced with vectors containing combinations of fluorescent and luciferase reporter genes under constitutive cytomeglavirus (CMV) or chondrocyte-lineage (Col2) promoters. Reporter...
全文获取路径: European Cells and Materials (eCM)  (合作)
影响因子:4.558 (2012)

  • reporter 报告生成程序
  • luciferase 荧光素酶
  • constructs 建筑图纸生成系统
  • activity 活度
  • equine 马的
  • broadly 概括地
  • chondrocyte 软骨细胞
  • heterogeneity 不均匀性
  • expression 表示
  • lineage 血统