Genetically encoded FRET probes for direct mapping and quantification of intracellular oxygenation level via fluorescence lifetime imaging
作者: Alessio AndreoniRozhin PenjweiniBranden RoarkeMarie-Paule StrubDan L. SackettJay R. Knutson
作者单位: National Institutes of Health (United States);;Univ. of Virginia (United States);;Massachusetts Institute of Technology (United States);;JenLab GmbH (Germany)
论文集英文名称: BiOS
来源数据库: SPIE-the International Society for Optical Engineering
DOI: 10.1117/12.2510646
原始语种摘要: Molecular oxygen is an important reporter of metabolic and physiological status at the cellular and tissue level, and its concentration is used for the evaluation of many diseases (e.g.: cancer, coronary artery disease). The development of accurate and quantitative methods to measure O2 concentration ([O2]) in living cells, tissues and organisms is challenging and is subject of intense research. We developed a protein-based, fluorescent oxygen sensor that can be expressed directly in cells to monitor [O2] in the intracellular environment. We fused Myoglobin (Myo), a physiological oxygen carrier, with mCherry, a fluorescent protein, to build a fluorescence resonance energy transfer (FRET) pair, Myo-mCherry. The changes in the spectral properties of Myoglobin upon...
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关键词翻译
关键词翻译
  • probes 探针
  • quantification 量化
  • fluorescence 荧光
  • fluorescent 萤光的
  • oxygenation 加氧酌
  • encoded 编码
  • concentration 浓度
  • level 水准
  • available 可供应的
  • lifetime 寿命