DsbC chaperone mediated soluble expression of human TNF-á in E. coli
作者: Hassan DariushnejadSafar FarajniaNosratollah ZargamiShiva Ahdi KhosroshahiLeila Rahbarnia
刊名: Minerva Biotecnologica, 2018, Vol.30 (1)
来源数据库: ProQuest Journal
原始语种摘要: BACKGROUND: TNF-α is a pleiotropic cytokine that plays an important role in both physiological and pathological conditions. An increasing demand of large amount of bioactive TNF for use in clinic and drug development strategies make this cytokine as one of the most important biotechnologically produced pharmaceuticals. In this study, soluble expression of TNF-α in cytoplasm of E. coli was attempted using DsbC chaperon and the effects of environmental condition including inducer concentration and incubation temperature was evaluated. METHODS: The gene encoding TNF-α was isolated by PCR reaction from cDNA that generated from PBMC total RNA and cloned into the pET-22b expression vector. Two type of E. coli strains, IPTG concentrations and growth temperatures as well as different type of...
全文获取路径: ProQuest  (合作)
影响因子:0.379 (2012)

  • coli 大肠菌
  • expression 表示
  • soluble 可溶的
  • amount 总计
  • active 活动的
  • concentration 浓度
  • recombinant 重组体
  • important 重要的
  • incubation 孵化
  • strain 应变