Gene Knockout Protocols
作者: Susanne FeilNadejda ValtchevaRobert Feil
英文丛书称: Methods in Molecular Biology
出版社: Humana Press,   2009
ISBN: 978-1-934115-26-8
来源数据库: Springer Book
DOI: 10.1007/978-1-59745-471-1_18
关键词: Transgenic miceinducible gene knockoutsomatic mutagenesisCreER recombinasetamoxifenin uteroROSA26R26RX-Gal stainingmouse models of human disease
英文摘要: AbstractThe Cre/lox site-specific recombination system has emerged as an important tool for the generation of conditional somatic mouse mutants. This method allows one to control gene activity in space and time in almost any tissue of the mouse, thus opening new avenues for studying gene function and for establishing sophisticated animal models of human diseases. A major technical advance in terms of in vivo inducibility was the development of ligand-dependent Cre recombinases that can be activated by administration of tamoxifen to the animal. Here we describe how tamoxifen-dependent Cre recombinases, so-called CreER recombinases, work and how they can be used to generate time- and tissue-specific mouse mutants. The focus will be on the CreERT2 recombinase, which is currently the most...
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关键词翻译
关键词翻译
  • tamoxifen 他莫西芬
  • recombinase 重组酶
  • somatic 躯体的
  • mouse 小鼠
  • mutagenesis 突变形成
  • generation 世代
  • recombination 再化合
  • available 可供应的
  • knockout 出模器
  • reporter 报告生成程序