Expression Vectors for the Rapid Purification of Recombinant Proteins in Bacillus subtilis
作者: Hoang Duc NguyenTrang Thi Phuong PhanWolfgang Schumann
作者单位: 1Institute of Genetics, University of Bayreuth
刊名: Current Microbiology, 2007, Vol.55 (2), pp.89-93
来源数据库: Springer Nature Journal
DOI: 10.1007/s00284-006-0419-5
英文摘要: Abstract(#br)We describe the construction of six novel plasmid-based IPTG-inducible expression vectors for Bacillus subtilis and related species. While one vector allows intracellular production of recombinant proteins, the second provides a strong secretion signal. The third vector allows addition of the c -Myc epitope tag, and the remaining three vectors provide the purification tags His and Strep. The versatility of all six vectors was demonstrated by the insertion of several reporter genes and by their regulated overexpression. Recombinant proteins with a His- or Strep-tag could be purified to near homogeneity in a single step.
全文获取路径: Springer Nature  (合作)
影响因子:1.52 (2012)

  • subtilis 微小
  • Rapid 拉皮德牌照相机