Delivery of foreign genes to intact barley cells by high-velocity microprojectiles
作者: R. R. MendelB. MüllerJ. SchulzeV. KolesnikovA. Zelenin
作者单位: 1Zentralinstitut für Genetik und Kulturpflanzenforschung, Academy of Sciences of GDR
2Institut für Züchtungsforschung, Academy of Agricultural Sciences of GDR
3Institute of Molecular Biology, USSR Academy of Sciences
刊名: Theoretical and Applied Genetics, 1989, Vol.78 (1), pp.31-34
来源数据库: Springer Nature Journal
DOI: 10.1007/BF00299749
关键词: Gene transferBarley ( Hordeum vulgare L.)Suspension culture cellsParticle accelerationTransient expression
英文摘要: Summary(#br)Foreign DNA was introduced through the cell walls of intact suspension culture cells of barley ( Hordeum vulgare L.) by utilizing the particle acceleration approach. DNA-coated microscopic tungsten particles were accelerated to velocities that permitted their penetration of intact cells. Chimaeric constructs of β -glucuronidase and neomycin phosphotransferase II under the control of the dual Agrobacterium T R 1′2′ promoter or the cauliflower mosaic virus 35S promoter served as reporter genes. Three days after particle delivery, high-level expression of both reporter genes was observed. That plasmid size could be critical for stabilizing DNA in the course of particle delivery will be discussed.
全文获取路径: Springer Nature  (合作)
影响因子:3.658 (2012)

  • barley 大麦
  • intact 完整的
  • velocity 速度
  • cells 麻风细胞
  • foreign 外国的