Nuclear transplantation in different strains of zebrafish
作者: Wei HuYaping WangShangping ChenZuoyan Zhu
作者单位: 1State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences
刊名: Chinese Science Bulletin, 2002, Vol.47 (15), pp.1277-1280
来源数据库: Springer Journal
DOI: 10.1360/02tb9283
关键词: Zebrafishdifferent strainsnuclear transplantationDNA fingerprint
英文摘要: Abstract(#br)Single later blastula nuclei from AB strain of zebrafish ( Danio rerio ) were transplanted into enucleated unfertilized eggs of Long fin strain. Of 1119 cloning embryos, 14 reconstructed embryos developed into fry. DNA fingerprinting systems of the cloned fish were similar to those of the nuclear donor fish, but were distinctly different from those of the nuclear recipient fish. It confirmed that the genetic material originated from nuclear donor cell other than from nuclear recipient egg. The research suggested that the basic technique for nuclear transplantation performed with different strains of zebrafish has made a breakthrough. It should be helpful for the study of some important developmental problems such as gene function, the regulation ogene expression during animal...
全文获取路径: Springer  (合作)
影响因子:1.319 (2012)

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