Unintended consequence of plant transformation: biolistic transformation caused transpositional activation of an endogenous retrotransposon Tos17 in rice ssp. japonica cv. Matsumae
作者: R. WuW. L. GuoX. R. WangX. L. WangT. T. ZhuangJihong Liu ClarkeB. Liu
作者单位: 1Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, Northeast Normal University
2Plant Health and Protection Division, Norwegian Institute for Agricultural and Environmental Research
刊名: Plant Cell Reports, 2009, Vol.28 (7), pp.1043-1051
来源数据库: Springer Journal
DOI: 10.1007/s00299-009-0704-4
关键词: Genetic transformationParticle bombardmentTos17Transpositional activityDNA methylationRice
英文摘要: Abstract(#br)Genetic instability could be provoked as an unintended consequence of genetic engineering in plants. Here, we report that the rice endogenous long terminal repeat (LTR) retrotransposon Tos17 was transpositionally activated only in transgenic calli and their regenerated plants produced by biolistic transformation in rice ( Oryza sativa L.) ssp. japonica cv. Matsumae. Moreover, the transpositional activity of Tos17 was sustained after plant regeneration in the T0 generation, and produced new germinal insertions. In contrast, the element remained totally quiescent in calli and regenerated plants from tissue culture of this genotype. Nonetheless, transcriptional induction and cytosine demethylation of Tos17 were found to have occurred with no significant difference in both kinds...
全文获取路径: Springer  (合作)
影响因子:2.509 (2012)

  • plant 植物
  • transformation 变换
  • endogenous 内成的
  • consequence 后承
  • activation 活化