Validation of a high-throughput microtissue fabrication process for 3D assembly of tissue engineered cartilage constructs
作者: B. S. SchonK. SchrobbackM. VenS. StroebelG. J. HooperT. B. F. Woodfield
作者单位: 1Christchurch Regenerative Medicine and Tissue Engineering (CReATE) Group, Department of Orthopaedic Surgery, University of Otago Christchurch
2Institute of Health & Biomedical Innovation, Queensland University of Technology
3MIRA, Institute for Biomedical Technology & Technical Medicine, University of Twente
刊名: Cell and Tissue Research, 2012, Vol.347 (3), pp.629-642
来源数据库: Springer Nature Journal
DOI: 10.1007/s00441-011-1311-6
关键词: CartilagePelletTissue assemblyMicrotissueRepair
英文摘要: Abstract(#br)Described here is a simple, high-throughput process to fabricate pellets with regular size and shape and the assembly of pre-cultured pellets in a controlled manner into specifically designed 3D plotted porous scaffolds. Culture of cartilage pellets is a well-established process for inducing re-differentiation in expanded chondrocytes. Commonly adopted pellet culture methods using conical tubes are inconvenient, time-consuming and space-intensive. We compared the conventional 15-mL tube pellet culture method with 96-well plate-based methods, examining two different well geometries (round- and v-bottom plates). The high-throughput production method was then used to demonstrate guided placement of pellets within a scaffold of defined pore size and geometry for the 3D assembly...
全文获取路径: Springer Nature  (合作)
影响因子:3.677 (2012)

  • assembly 组装
  • fabrication 制造
  • throughput 吞吐量
  • constructs 建筑图纸生成系统
  • process 过程
  • engineered 设计的
  • cartilage 软骨
  • tissue 组织