Post-column labeling techniques in amino acid analysis by liquid chromatography
作者: Pantelis G. Rigas
作者单位: 1Biotoxins and Analytical Chemistry Laboratory, Department of Fisheries and Aquaculture Technology, Alexander Technological Educational Institute of Thessaloniki
刊名: Analytical and Bioanalytical Chemistry, 2013, Vol.405 (25), pp.7957-7992
来源数据库: Springer Nature Journal
DOI: 10.1007/s00216-013-7127-3
关键词: Amino acid analysisDerivatizationPost-column labelingLiquid chromatography
原始语种摘要: Abstract(#br)Amino acid analysis (AAA) has always presented an analytical challenge in terms of sample preparation, separation, and detection. Because of the vast number of amino acids, various separation methods have been applied taking into consideration the large differences in their chemical structures, which span from nonpolar to highly polar side chains. Numerous separation methods have been developed in the past 60 years, and impressive achievements have been made in the fields of separation, derivatization, and detection of amino acids (AAs). Among the separation methods, liquid chromatography (LC) prevailed in the AAA field using either pre-column or post-column labeling techniques in order to improve either separation of AAs or selectivity and sensitivity of AAA. Of the two...
全文获取路径: Springer Nature  (合作)
影响因子:3.659 (2012)

  • liquid 液体
  • chromatography 色层分离法
  • amino 氨基
  • column 
  • analysis 分析
  • labeling 加标