Purification and Characterization of a Hemocyanin (Hemo1) with Potential Lignin-Modification Activities from the Wood-Feeding Termite, Coptotermes formosanus Shiraki
| 作者： | Haifang Qiu, Alei Geng, Daochen Zhu, Yilin Le, Jian Wu, Nienwen Chow, J. H. David Wu, Jianzhong Sun |
| 作者单位： |
1Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University 2Department of Chemical Engineering, University of Rochester |
| 刊名： | Applied Biochemistry and Biotechnology, 2015, Vol.175 (2), pp.687-697 |
| 来源数据库： | Springer Journal |
| DOI： | 10.1007/s12010-014-1326-z |
| 关键词： | Hemocyanin; Oxidase; Pretreatment; Coptotermes formosanus; |
| 英文摘要： | Abstract(#br) Coptotermes formosanus Shiraki is a well-known wood-feeding termite, which can degrade not only cellulose and hemicellulose polysaccharides, but also some aromatic lignin polymers with its enzyme complex to the woody biomass. In this study, a very abundant protein was discovered and purified, using a three-step column chromatography procedure, from the tissue homogenate of the salivary glands and the gut of C. formosanus . Mass spectrometric analysis and the following peptide searching against the mRNA database toward this termite species indicated that the novel protein was a hemocyanin enzyme, termed as Hemo1, which further exhibited a strong oxidase activity in the substrate bioassays toward ABTS [2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)], as well as other... |
