Purification and Characterization of a Hemocyanin (Hemo1) with Potential Lignin-Modification Activities from the Wood-Feeding Termite, Coptotermes formosanus Shiraki
作者: Haifang QiuAlei GengDaochen ZhuYilin LeJian WuNienwen ChowJ. H. David WuJianzhong Sun
作者单位: 1Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University
2Department of Chemical Engineering, University of Rochester
刊名: Applied Biochemistry and Biotechnology, 2015, Vol.175 (2), pp.687-697
来源数据库: Springer Journal
DOI: 10.1007/s12010-014-1326-z
关键词: HemocyaninOxidasePretreatmentCoptotermes formosanus
英文摘要: Abstract(#br) Coptotermes formosanus Shiraki is a well-known wood-feeding termite, which can degrade not only cellulose and hemicellulose polysaccharides, but also some aromatic lignin polymers with its enzyme complex to the woody biomass. In this study, a very abundant protein was discovered and purified, using a three-step column chromatography procedure, from the tissue homogenate of the salivary glands and the gut of C. formosanus . Mass spectrometric analysis and the following peptide searching against the mRNA database toward this termite species indicated that the novel protein was a hemocyanin enzyme, termed as Hemo1, which further exhibited a strong oxidase activity in the substrate bioassays toward ABTS [2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)], as well as other...
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影响因子:1.893 (2012)

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关键词翻译
关键词翻译
  • Termite 特米特铅基轴承合金
  • Lignin 木质素