Roles of ionotropic glutamate receptors in early developing neurons derived from the P19 mouse cell line
作者: Yi-Hsuan LeeChun-Hua LinLi-Wen HsuSsu-Yao HuWen-Te HsiaoYuan-Soon HO
作者单位: 1Department of Physiology, Taipei Medical University
2Graduate Institute of Medical Sciences, Taipei Medical University
3Institute of Biotechnology, Taipei Medical University
刊名: Journal of Biomedical Science, 2003, Vol.10 (2), pp.199-207
来源数据库: Springer Nature Journal
DOI: 10.1007/BF02256055
关键词: P19 cellsGlutamate receptorKainic acidHypoxiaNerve growth factor
原始语种摘要: Abstract(#br)We cultured a P19 mouse teratocarcinoma cell line and induced its neuronal differentiation to study the function of ionotropic glutamate receptors (GluRs) in early neuronal development. Immunocytochemical studies showed 85% neuronal population at 5 days in vitro (DIV) with microtubule-associated protein 2-positive staining. Thirty percent and 50% of the cells expressed the α-amino-3-hydroxy-5-methyl-4-isopropinonate (AMPA) receptor subunit, GluR2/3, and the kainate (kainic acid; KA) receptor subunit, GluR5/6/7, respectively. In Western blot analysis, the temporal expression of GluR2/3 began to appear at 3 DIV, whereas GluR5/6/7 was already expressed in the undifferentiated cells. P19-derived neurons began to respond to glutamate, AMPA and KA, but not to the metabotropic GluR...
全文获取路径: Springer Nature  (合作)
影响因子:2.458 (2012)

  • developing 显影
  • ionotropic 离子移变的
  • mouse 小鼠
  • derived 导生的
  • glutamate 谷氨酸盐酯或根
  • early