Ultrasound microbubble-mediated delivery of the siRNAs targeting MDR1 reduces drug resistance of yolk sac carcinoma L2 cells
作者: Yun HeYang BiYi HuaDongyao LiuSheng WenQiang WangMingyong LiJing ZhuTao LinDawei HeXuliang LiZhigang WangGuanghui Wei
作者单位: 1The Children's Hospital of Chongqing Medical University
2Chongqing Medical University, Ministry of Education
3the Second Affiliated Hospital of Chongqing Medical University
刊名: Journal of Experimental & Clinical Cancer Research, 2011, Vol.30 (1)
来源数据库: Springer Journal
DOI: 10.1186/1756-9966-30-104
关键词: Yolk sac carcinomaUltrasound therapyRNA interferenceMultiple drug resistance geneTransfection
英文摘要: Abstract(#br) Background(#br)MDR1 gene encoding P-glycoprotein is an ATP-dependent drug efflux transporter and related to drug resistance of yolk sac carcinoma. Ultrasound microbubble-mediated delivery has been used as a novel and effective gene delivery method. We hypothesize that small interfering RNA (siRNA) targeting MDR1 gene (siMDR1) delivery with microbubble and ultrasound can down-regulate MDR1 expression and improve responsiveness to chemotherapeutic drugs for yolk sac carcinoma in vitro .(#br) Methods(#br)Retroviral knockdown vector pSEB-siMDR1s containing specific siRNA sites targeting rat MDR1 coding region were constructed and sequence verified. The resultant pSEB-siMDR1 plasmids DNA were encapsulated with lipid microbubble and the DNA release were triggered by ultrasound...
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  • microbubble 微泡
  • targeting 导向目标
  • carcinoma 癌症
  • cells 麻风细胞
  • delivery 移交
  • resistance 抵抗