Improved extracellular expression and high-cell-density fed-batch fermentation of chitosanase from Aspergillus Fumigatus in Escherichia coli
作者: Liang HuangQinhong WangSijing JiangYuling ZhouGuimin ZhangYanhe Ma
作者单位: 1Hubei University
2Chinese Academy of Science
刊名: Bioprocess and Biosystems Engineering, 2016, Vol.39 (11), pp.1679-1687
来源数据库: Springer Journal
DOI: 10.1007/s00449-016-1643-4
关键词: ChitosanaseSecretory expressionFed-batch fermentation
英文摘要: Abstract(#br)Chitosanase (CSN) from Aspergillus fumigatus has good thermal stability, wide pH range duration, and effective hydrolysis for chitosan. Inhere, CSN was successfully expressed in Escherichia coli followed by extracellular secretion under the guidance of an N-terminal signal peptide PelB, which effectively prompted its secretion out of E. coli cells. To facilitate its later purification, N-terminal or C-terminal 6xHis epitope tag was added to the PelB-CSN protein complex. Our results indicated that PelB-CSN without 6xHis-tag (PelB-CSN) or with N-terminal 6xHis-tag (PelB-CSN-N) can both be effectively secreted into the medium, while CSN with 6xHis-tag anchored at C-terminus was expressed as inclusion bodies. Process optimization strategies were further developed to improve the...
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影响因子:1.869 (2012)

  • batch 一批
  • fermentation 发酵
  • density 密度
  • Escherichia 埃希氏菌属
  • expression 表示
  • extracellular 胞外的