Production of Cyclodextrins from Cornstarch Granules in a Sequential Batch Mode and in the Presence of Ethanol
作者: Luiza Pedrina Vilxenski CalsavaraAlessandro Rogério Dias da CunhaTiago Albertini BalbinoGisella Maria ZaninFlavio Faria de Moraes
作者单位: 1State University of Maringá
刊名: Applied Biochemistry and Biotechnology, 2011, Vol.165 (7-8), pp.1485-1493
来源数据库: Springer Nature Journal
DOI: 10.1007/s12010-011-9369-x
关键词: CyclodextrinProductionStarchCGTaseEthanolSequential batch
原始语种摘要: Abstract(#br)The influence of Toruzyme® cyclomaltodextrin glucanotransferase concentration and the presence of ethanol have been studied for the production of α-, β-, and γ-cyclodextrins (CDs) from 15% ( w / v ) cornstarch, at 65 °C and pH 6, with the aim of increasing CD yield. The selected concentrations for a single batch reactor were 10% ( v / v ) ethanol and 0.1% ( v / v ) enzyme, yielding after 12 h, 37% total CDs, of which 52.2% was α-CD, 38.8% β-CD, and 9.0% γ-CD. The enzyme specific activities per unit mass of protein for producing α-, β-, and γ-CD were 37.25, 19.61, and 8.63 U mg−1, respectively. Total CD yield per milliliter of enzyme was 55 g. To increase CD yield per enzyme charge and thus reduce costs, the production of CDs was tested with two sequential batches...
全文获取路径: PDF下载  Springer Nature  (合作)
影响因子:1.893 (2012)