Functional characterization of BC039389-GATM and KLK4-KRSP1 chimeric read-through transcripts which are up-regulated in renal cell cancer
作者: Dorothee PfluegerChristiane MittmannSilvia DehlerMark A RubinHolger MochPeter Schraml
作者单位: 1University Hospital Zurich
2ETH and University of Zurich
3Institute of Precision Medicine of Weill Cornell Medical College and New York-Presbyterian Hospital
4Weill Cornell Medical College
刊名: BMC Genomics, 2015, Vol.16 (1)
来源数据库: Springer Journal
DOI: 10.1186/s12864-015-1446-z
关键词: RNA-SeqRenal cell carcinomaRead-throughGATMKLK4Chimera
英文摘要: Abstract(#br) Background(#br)Chimeric read-through RNAs are transcripts originating from two directly adjacent genes (<10 kb) on the same DNA strand. Although they are found in next-generation whole transcriptome sequencing (RNA-Seq) data on a regular basis, investigating them further has usually been refrained from. Therefore, their expression patterns or functions in general, and in oncogenesis in particular, are poorly understood.(#br) Results(#br)We used paired-end RNA-Seq and a specifically designed computational data analysis pipeline (FusionSeq) to nominate read-through events in a small discovery set of renal cell carcinomas (RCC) and confirmed them in a larger validation cohort.(#br)324 read-through events were called overall; 22/27 (81%) selected nominees passed validation with...
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影响因子:4.397 (2012)

  • regulated 规定
  • renal 肾的
  • which 哪个
  • cancer 癌症
  • through 经过