Development of P grac 100-based expression vectors allowing high protein production levels in Bacillus subtilis and relatively low basal expression in Escherichia coli
作者: Trang Thi Phuong PhanLinh Thuoc TranWolfgang SchumannHoang Duc Nguyen
作者单位: 1VNUHCMC-University of Science
2Universität of Bayreuth
刊名: Microbial Cell Factories, 2015, Vol.14 (1)
来源数据库: Springer Nature Journal
DOI: 10.1186/s12934-015-0255-z
关键词: Bacillus subtilis 1012IPTG-inducible promoterP gracP grac 100pHT253pHT254pHT255
英文摘要: Abstract(#br) Background(#br)In general, fusion of recombinant genes to strong inducible promoters allowing intracellular expression in Bacillus subtilis is a two-step process. The ligation products are transformed into Escherichia coli , followed by identification of the correct plasmid, and this plasmid is subsequently transformed into B. subtilis . This raises the problem that basal level of expression of the recombinant gene could be harmful for E. coli cells. Based on the P grac promoter, we optimized the UP element, the -35, 15, -10 and the +1 region to enhance the promoter activity in B. subtilis after induction. However, detailed investigations for a promoter to develop expression vectors that allows high protein production levels in B. subtilis and a relatively low basal...
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影响因子:3.306 (2012)

  • expression 表示
  • Escherichia 埃希氏菌属
  • protein 蛋白质
  • relatively 比较
  • subtilis 微小
  • production 生产
  • based 基于
  • basal 基部