Determination of Zearalanol and Its Analog Zearalanone in Muscle Tissues by Amplified Luminescent Proximity Homogeneous Assay (AlphaLISA)
作者: Qian GongCunxian XiFuping NieShurui CaoBobin TangLei ZhangXianliang LiGuoming WangDongdong ChenZhaode Mu
作者单位: 1College of Pharmacy, Chongqing Medical University
2Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology
3Chongqing Entry-Exit Inspection and Quarantine Bureau, Chongqing Engineering Technology, Research Center of Import and Export Food Safety
4Chinese Academy of Inspection and Quarantine
刊名: Food Analytical Methods, 2017, Vol.10 (2), pp.339-346
来源数据库: Springer Nature Journal
DOI: 10.1007/s12161-016-0586-3
关键词: AlphaLISAZearalanolZearalanoneHomogeneous
英文摘要: Abstract(#br)A homogenous light-induced chemiluminescence immunoassay (AlphaLISA) method was established for the determination of residues of zearalanol and its analog zearalanone in muscle tissue samples. AlphaLISA is a bead-based proximity assay. When donor and acceptor beads proximity, a cascade of chemical reactions begin. The end result is a greatly amplified signal that contributes to the detection sensitivity down to the attomole level. Compared with other methods, the AlphaLISA has characteristics of homogeneity, being free of cleaning, high sensitivity. The method showed a linear relationship in the range of 0.01–4 ng/mL ( R 2 > 0.99); the sensitivity of the assay was 0.066 ng/mL. Cross-reactivity rate of zearalanol was 100 % and zearalanone was 82.1 %, and other compounds were...
全文获取路径: Springer Nature  (合作)
影响因子:1.969 (2012)