Reconstruction and analysis of the genome-scale metabolic model of schizochytrium limacinum SR21 for docosahexaenoic acid production
作者: Chao YeWeihua QiaoXiaobin YuXiaojun JiHe HuangJackie L. CollierLiming Liu
作者单位: 1Jiangnan University
2Nanjing Tech University
3Stony Brook University
刊名: BMC Genomics, 2015, Vol.16 (1)
来源数据库: Springer Journal
DOI: 10.1186/s12864-015-2042-y
关键词: Schizochytrium limacinum SR21Docosahexaenoic acidGenome-scale metabolic modelPolyketide synthase systemMinimization of metabolic adjustment algorithm
英文摘要: Abstract(#br) Background(#br) Schizochytrium limacinum SR21 is a potential industrial strain for docosahexaenoic acid (DHA) production that contains more than 30–40 % DHA among its total fatty acids.(#br) Methods(#br)To resolve the DHA biosynthesis mechanism and improve DHA production at a systematic level, a genomescale metabolic model (GSMM), named iCY1170_DHA, which contains 1769 reactions, 1659 metabolites, and 1170 genes, was reconstructed.(#br) Results(#br)Based on genome annotation results and literature reports, a new DHA synthesis pathway based on a polyketide synthase (PKS) system was detected in S. limacinum . Similarly to conventional fatty acid synthesis, the biosynthesis of DHA via PKS requires abundant acetyl-CoA and NADPH. The in silico addition of malate and citrate led...
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影响因子:4.397 (2012)

  • metabolic 新陈代谢的
  • analysis 分析
  • scale 度盘
  • genome 基因组
  • production 生产
  • model 模型