Time course of foreign gene integration and expression in transgenic fish embryos
作者: Haobin ZhaoShangping ChenYonghua SunYaping WangZuoyan Zhu
作者单位: 1State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences
刊名: Chinese Science Bulletin, 2000, Vol.45 (8), pp.734-739
来源数据库: Springer Journal
DOI: 10.1007/BF02886180
关键词: gene integration and expressiontime coursenuclear transplantationfishGFP
英文摘要: Abstract(#br)Using a nuclear transplantation approach, the integration and expression of the green fluorescent protein ( GFP ) gene in the embryogenesis of transgenic loach ( Misgurnus anguillicaudatus Cantor) have been studied. The GFP gene expression is first observed at the gastrula stage, which is consistent with the initiation of cell differentiation of fish embryos. The time course of the foreign gene expression is correlated with the regulatory sequences. The expression efficiency also depends on the gene configuration: the expression of pre-integrating circular plasmid at early embryos is higher than that of the linear plasmid. The integration of the GFP gene is first detected at the blastula stage and lasts for quite a long period. When two types of different plasmids are...
全文获取路径: Springer  (合作)
影响因子:1.319 (2012)

  • integration 集成
  • course 航向
  • expression 表示
  • foreign 外国的